NCRO Biotools
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Internal tools

Design the assay before it reaches the bench.

Access is restricted to authorised NCRO staff and partners. Every tool runs in your browser and uses NCRO's own gene, pricing and reference data. Designs are starting points: verify primers in silico and validate them in the wet lab before use.

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Solution Designer

A guided workflow that turns "what do you need to test?" into an itemized, priced NGS, PCR or Sanger order. There are three ways in (by target, by application or by technique) plus a quick route for Sanger or PCR of specific exons, where you click the exact exons on a map and see the amplicon count before continuing.

Best for: quotes, scoping a client request, exon-level Sanger/PCR orders
Did you know? Frederick Sanger's 1977 chain-termination method is still the accuracy benchmark labs use to confirm a variant found by NGS.
Open Solution Designer →

Gene Panel Designer

Pick targets by clinical application in the Target Planner (NCCN oncology panels, β-thalassaemia, CFTR, hereditary breast cancer, pharmacogenomics, PIK3CA/KRAS hotspot assays), then design Sanger, NGS amplicon, HRM or ARMS primers. Includes SNP-under-primer and genome-wide specificity checks.

Best for: primer design, multiplex NGS panels, HRM / ARMS genotyping
Did you know? Kary Mullis conceived PCR on a night drive in 1983. It won him the 1993 Nobel Prize in Chemistry.
Open Gene Panel Designer →

Species ID PCR Designer

Conventional and TaqMan primer/probe design for meat and seafood species identification and adulteration panels. It fetches reference sequences, designs, cross-checks and pools multiplexes automatically.

Best for: halal and food-fraud screening, seafood authentication
Did you know? In 2003 Paul Hebert proposed a short stretch of the COI gene as a "barcode" for animal species, and it is now standard in food authentication.
Open Species ID PCR Designer →

Potency Assay Designer

Build a cell-based potency testing workflow, with costing, for a biologic or biosimilar.

Best for: biopharma release and comparability testing
Did you know? Köhler and Milstein's 1975 hybridoma method for monoclonal antibodies, the ancestor of today's biologic drugs, won the 1984 Nobel Prize.
Open Potency Assay Designer →

Sequence Aligner

Align Sanger reads or references. Load an exon straight from hg38, align forward and reverse reads (auto-orientation), and get a list of differences, including heterozygous calls, in genomic coordinates.

Best for: Sanger variant confirmation, comparing references
Did you know? Needleman and Wunsch published their alignment algorithm in 1970. It was the first dynamic-programming method in biology.
Open Sequence Aligner →

While you're here

More stories about the tools and labs behind modern genomics: Lab Stories.